Leuconostoc mesenteroides M13: optimization of bioprocesses and antioxidant property for food industry application

Heloísa Carneiro COLARES; Vinícius Souza TARABAL; Hiure Gomes Ramos MEIRA; Daniela Regiane da SILVA; Yuri Komatsu Damas ABUD; Celso Barbosa de SANT’ANNA FILHO; Adriano Guimarães PARREIRA; Vanessa Faria CORTES; Jose Antonio da SILVA; José Mauro GRANJEIRO; Juliana Teixeira de MAGALHÃES; Daniel Bonoto GONÇALVES; Paulo Afonso GRANJEIRO

doi:10.5327/fst.00423

Autores

Heloísa Carneiro COLARES Universidade Federal de São João del-Rei, Biotechnological Processes and Macromolecules Purification Laboratory, Divinópolis, Minas Gerais, Brazil. https://orcid.org/0000-0002-3354-5774
Vinícius Souza TARABAL Universidade Federal de São João del-Rei, Biotechnological Processes and Macromolecules Purification Laboratory, Divinópolis, Minas Gerais, Brazil. https://orcid.org/0000-0002-8736-8531
Hiure Gomes Ramos MEIRA Universidade Federal de São João del-Rei, Biotechnological Processes and Macromolecules Purification Laboratory, Divinópolis, Minas Gerais, Brazil. https://orcid.org/0000-0001-8563-3170
Daniela Regiane da SILVA Universidade Federal de São João del-Rei, Biotechnological Processes and Macromolecules Purification Laboratory, Divinópolis, Minas Gerais, Brazil. https://orcid.org/0009-0005-8781-2830
Yuri Komatsu Damas ABUD Instituto Nacional de Metrologia, Qualidade e Tecnologia, Duque de Caxias, Rio de Janeiro, Brazil. https://orcid.org/0000-0002-3561-4817
Celso Barbosa de SANT’ANNA FILHO Instituto Nacional de Metrologia, Qualidade e Tecnologia, Duque de Caxias, Rio de Janeiro, Brazil. https://orcid.org/0000-0001-9759-9512
Adriano Guimarães PARREIRA Universidade Federal de São João del-Rei, Biotechnological Processes and Macromolecules Purification Laboratory, Divinópolis, Minas Gerais, Brazil. https://orcid.org/0000-0002-7770-7165
Vanessa Faria CORTES Universidade Federal de São João del-Rei, Laboratory of Cellular Biochemistry, Divinópolis, Minas Gerais, Brazil. https://orcid.org/0000-0002-6464-7634
Jose Antonio da SILVA Universidade Federal de São João del-Rei, Biotechnological Processes and Macromolecules Purification Laboratory, Divinópolis, Minas Gerais, Brazil. https://orcid.org/0000-0001-9134-1211
José Mauro GRANJEIRO Instituto Nacional de Metrologia, Qualidade e Tecnologia, Duque de Caxias, Rio de Janeiro, Brazil. https://orcid.org/0000-0002-8027-8293
Juliana Teixeira de MAGALHÃES Universidade Federal de São João del-Rei, Microbiology Laboratory, Divinópolis, Minas Gerais, Brazil. https://orcid.org/0000-0002-0532-7323
Daniel Bonoto GONÇALVES Universidade Federal de São João del-Rei, Department of Biosystems Engineering, São João del-Rei, Minas Gerais, Brazil. https://orcid.org/0000-0002-8178-1026
Paulo Afonso GRANJEIRO Universidade Federal de São João del-Rei, Biotechnological Processes and Macromolecules Purification Laboratory, Divinópolis, Minas Gerais, Brazil. https://orcid.org/0000-0003-0322-0861

DOI:

https://doi.org/10.5327/fst.00423

Palavras-chave:

Probiotics, Bioprocess, Antioxidant, Shelf-life

Resumo

Several reactive oxygen species are related to physiological functions but also play roles in the development of certain human diseases. Reactive oxygen species are counterbalanced by a physiological defense network such as antioxidants. Probiotic strains have been used as natural antioxidants, but its viable scalability is a challenger. The objective of this work was to optimize the bioprocesses of Leuconostoc mesenteroides M13 and evaluate its antioxidant activity and its shelf-life. The optimized production of M13 biomass was 2,542% greater than non-optimized processes, resulting in a yield of 2.67 × 10¹⁰ CFU mL^-1 under conditions comprising 13.67 g L^-1 of yeast extract and .73 g L^-1 of magnesium sulfate. The aggregated spherical structure observed in scanning electron microscopy may have a certain protective effect. The viability of the lyophilized strain storage at 4°C was maintained for a period of up to 8 months. Antioxidant activity assays revealed efficacies for intact and lysed cells as 81 and 65%, respectively. The survival rate of the M13 strain in the presence of hydrogen peroxide was observed at 86% (1.0 mM for 8 h). M13 demonstrated a cost-effective strategy for probiotic application in the food industry as an antioxidant supplement.

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Leuconostoc mesenteroides M13: optimization of bioprocesses and antioxidant property for food industry application

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